Manchester City were the first club to pull out after Chelsea had signalled their intent to do so by preparing documentation to withdraw.
The other four sides – Arsenal, Liverpool, Manchester United and Tottenham – have all now followed suit.
Italian side Inter Milan are also set to withdraw as they no longer wish to be involved with the project.
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Manchester City confirmed they have “formally enacted the procedures to withdraw” from the Super League.
Liverpool said their involvement in the proposed breakaway league “has been discontinued”.
Manchester United said they had “listened carefully to the reaction from our fans, the UK government and other key stakeholders” in making their decision to not take part.
Arsenal apologised in an open letter to their fans and said they had “made a mistake”, adding they were withdrawing after listening to them and the “wider football community”.
Tottenham chairman Daniel Levy said the club regretted the “anxiety and upset” caused by the proposal.
Chelsea confirmed they have “begun the formal procedures for withdrawal from the group” that they only joined “late last week”.